Basic information[1]
The main components of the mouse phosphatidylserine (PS) ELISA kit: enzyme plate, reagents, standards, etc.
Purpose: Special reagent for scientific research and experiment, not for clinical diagnosis.
Specimens: serum, plasma, cell supernatant, urine, body fluids, lavage fluid, cerebrospinal cord, atrial fluid, thoracic fluid, tissue, etc.
Species: human, rat, mouse, rabbit, pig, dog, monkey, horse, cow, sheep, chicken, duck, fish, etc.
Technical principles[1]
(1). Immobilization of antigen or antibody and enzyme labeling of antigen or antibody.
(2). The antigen or antibody bound to the surface of the solid phase carrier still maintains its immunological activity.
(3). The antigen or antibody of enzyme-labeled matting powder retains both its immunological activity and its enzyme activity.
(4). The tested specimen reacts with the antigen or antibody on the surface of the solid phase carrier. The enzyme-labeled antigen or antibody is then chemically added and bound to the solid-phase carrier through reaction.
(5). At this time, the amount of enzyme on the solid phase is in a certain proportion to the amount of the test substance in the specimen.
(6). After adding the substrate for the enzymatic reaction, the substrate is catalyzed by the enzyme into a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so qualitative or quantitative analysis can be performed based on the depth of the color. . The assay method has high sensitivity (pg-ng/ml level) and good reproducibility. Based on immunological reactions, it is a highly sensitive test technology that combines the specific reactions of antigens and antibodies with the efficient catalytic effect of enzymes on substrates.
