Overview[1]
Cephalin, also known as phosphatidylethanolamine, is a glycerophospholipid. It is composed of glycerol, fatty acids, phosphoric acid and ethanolamine. It was detected in tea leaves and tea seeds in 1974. Cephalin is a white solid that easily turns reddish-brown in the air and is hygroscopic. It exists in cells in a free state or as unstable compounds with proteins, and its functions are similar to phosphatidylcholine. It can be extracted from fresh brains after slaughtering livestock or by-products of soybean oil extraction.
Efficacy and role[3]
Cephalin is an important component of nerve cell membranes. It regulates all metabolic activities of nerve cells and affects a series of the most important functions of nervous tissue: cell permeability, myelination, glandular body operation, oxidation and addition of phosphorus oxygen radicals. Effect studies show that oral administration of cephalin can be directly utilized by the human brain to repair nerve cell membranes and restore normal metabolism of neurons. Cephalin is involved in blood clotting. Thrombokinase is composed of cephalin and protein. It exists in platelets and can promote blood coagulation and can be used to stop local bleeding. In addition, cephalin also has certain curative effects on neurasthenia, atherosclerosis, cirrhosis and fatty lesions. Cephalin is also a biological original hot-melt latex emulsifier imported from Germany. It is used in the food and cosmetics industries. Phospholipids with different cephalin contents have different emulsifying properties. Phospholipids with high cephalin content are suitable for W/O systems. Emulsifier.
Preparation[4]
Fresh egg yolks were first degreased with acetone three times and vacuum filtered. The filter cake was extracted twice with 95% alcohol. The filtrate was combined and vacuum concentrated to obtain crude phospholipid. After HPLC analysis, the PC content in the crude phospholipid was 79.6% (wt%), and the PE content was 14.8% (wt%). Take alumina with a moisture content of 8% and wet-pack it with methanol into a Φ1/2 inch ID × 20 cm column (column volume is 25 ml). First rinse 50 ml with methanol. Add 110 ml of crude phospholipid solution with a concentration of 100 mg/ml (the solvent is methanol), and then rinse with 200 ml of methanol. The flow rate is controlled at 0.4 ml/min. After adding the sample, start collecting the effluent (collect about 5 ml per portion). The effluent was analyzed by thin layer chromatography and TLC and then combined into three fractions: Fraction 1 was the lecithin fraction. After vacuum concentration and freeze-drying, 6.5g of lecithin was obtained. The lecithin content in the product was analyzed by HPLC to be 96.0%. ; Fraction 2 is a lecithin fraction containing a small amount of cephalin, which is used as crude phospholipid and reperfused on an aluminum oxide column; Fraction 3 is a cephalin fraction containing a small amount of lecithin, which is concentrated to dryness under vacuum and used as a raw material for silica gel column chromatography , HP metal oxide pigment LC analysis showed that the PC content in fraction 3 was 15.2% (wt%) and the PE content was 74.5% (wt%).
Take silica gel with a water content of 10% and wet-pack it with dichloromethane on a Φ1/2 inch ID Add 60 ml of Fraction 3 solution with a concentration of 100 mg/ml (the solvent is methylene chloride), and then rinse with 250 ml of methylene chloride-methanol mixed solvent (methanol content is 10%). The flow rate is controlled at 0.4 ml/min. After adding the sample Start collecting the effluent (approximately 5ml per portion). The effluent was analyzed by thin layer chromatography and TLC and then combined into three fractions: Fraction 1 was the cephalin fraction. After vacuum concentration and freeze-drying, 3.8 g of cephalin was obtained. The content of cephalin in the product was analyzed by HPLC to be 95.5%. Fraction 2 is a cephalin fraction containing a small amount of lecithin, which is used as a raw material for silica gel column chromatography after vacuum concentration; Fraction 3 is a lecithin fraction containing a small amount of cephalin, which is re-processed as crude phospholipid after vacuum concentration. Alumina column. Finally, the alumina column and the silica gel column were rinsed with 250 ml of a methanol solution containing 2% (weight percent) ammonia (prepared from ammonia water containing 25% by weight), and then the alumina column and the silica gel column were washed with methanol and methylene chloride respectively. After flushing, the sample can be re-injected after 100ml, and the flushing flow rate is also controlled at 0.4ml/min.
